Written by Russell McAndrews   
Monday, 25 May 2015 22:23

1. Fix specimen by submerging in a 10% buffered formalin* solution for 48 hours.



2. Transfer to distilled water for 24 hours.



3. Dehydrate specimen through an ethanol series, (10%, 30%, 60%, 70%) for a period of 24 hours in each solution.



4. Store in a 70% ethanol.



* Suggested recipe for buffered formalin:


formaldehyde solution 100mL

Sodium acid phosphate

(NaH2PO4-H2O) 4.0g

Anhydrous disodium phosphate

(Na2HPO4) 6.5g


Check pH, adjust to 7.0 with NaOH or H3PO4 if necessary.  Dilute to final volume of 1.0 L.


NOTE: Formalin is combustible, an irritant and may cause cancer.  Use glove and adequate ventilation.  Store in flame proof cabinet.

Last Updated on Friday, 19 June 2015 13:07